GMAT Test Prep: CR-65491485 GMAT Critical Reasoning

Manatees, aquatic mammals inhabiting Florida's rivers and coastal waters, swim close to the surface and are frequently killed in collisions with boats. To address the problem, boat traffic in manatee-populated waters is being required to maintain very low speeds. Unfortunately, manatees are unable to hear low-pitched sounds and a boat's sound lowers in pitch as the boat slows. Therefore, this approach may in fact make things worse rather than better.

Which of the following, if true, casts most doubt on the conclusion?
Select one of the following answer choices